Human Procalcitonin Antibody

Catalog #: MAB83502 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB83502-100
MAB83502-SP

Human Procalcitonin ELISA Standard Curve.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Procalcitonin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Procalcitonin in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 952123

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Procalcitonin synthetic peptide
Accession # P01258

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Procalcitonin DuoSet ELISA Kit (Catalog # DY8350-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human Procalcitonin ELISA Standard Curve. Recombinant Human Procalcitonin protein was serially diluted 2-fold and captured by Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Procalcitonin

Human Procalcitonin (CALCA and Calcitonin) is 141 amino acids (aa) in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-38, human Procalcitonin shares 64% aa identity with mouse Procalcitonin.

Entrez Gene IDs

796 (Human); 12310 (Mouse); 24241 (Rat)

Alternate Names

CALC1; CALCA; Calcitonin 1; calcitonin gene-related peptide 1; CGRP; Katacalcin; Procalcitonin