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Human Procalcitonin Antibody

Catalog #: AF8350 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF8350
AF8350-SP
Detection of Human Procalcitonin by Western Blot.
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Human Procalcitonin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Procalcitonin in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Procalcitonin
Ala26-Gly117 (Asn57Asp)
Accession # P01258
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Procalcitonin antibody by Western Blot. View Larger

Detection of Human Procalcitonin by Western Blot. Western blot shows lysates of human thyroid cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Procalcitonin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Procalcitonin at approximately 15-16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Procalcitonin

Human Procalcitonin (CALCA and Calcitonin) is 141 aa in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide and Calcitonin part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-117, human Procalcitonin shares 80% aa identity with mouse Procalcitonin. 

Entrez Gene IDs
796 (Human); 12310 (Mouse); 24241 (Rat)
Alternate Names
CALC1; CALCA; Calcitonin 1; calcitonin gene-related peptide 1; CGRP; Katacalcin; Procalcitonin

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Human Procalcitonin Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human