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Human Prominin 2 Antibody

Catalog #: MAB2024
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2024
MAB2024-SP
Prominin 2 in HepG2 Human Cell Line.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Prominin 2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Prominin 2. Stains human Prominin 2-transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 244029
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Prominin 2
Ala27-Leu834
Accession # AAM10541
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
LNCaP human prostate cancer cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Prominin 2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Prominin 2 in HepG2 Human Cell Line. Prominin 2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Prominin 2 Monoclonal Antibody (Catalog # MAB2024) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prominin 2

Prominin 2 is a pentaspan membrane glycoprotein predominantly expressed in neuroepithelial cells, hematopoietic stem cells and epithelial cells of the adult kidney and digestive tract (1, 2). Prominin 2 is a 112-kDa glycoprotein structurally related to prominin 1 (CD133). The amino acid identity between prominin 1 and prominin 2 is low (<30%), but they exhibit some redundant functions.

References
  1. Fargeas, C.A. et al. (2003) J. Biol. Chem. 278(10):8586.
  2. Fargeas, C.A. et al. (2003) Stem Cells 21(4):506.
Entrez Gene IDs
150696 (Human); 192212 (Mouse); 192211 (Rat)
Alternate Names
hPROML2; MGC138714; PROM2; PROM-2; Prominin 2; prominin-2; Prominin-like protein 2; prominin-related protein; PROML2

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Citation for Human Prominin 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Senescent Kidney Cells in Hypertensive Patients Release Urinary Extracellular Vesicles.
    Authors: Santelli A, Sun I, Eirin A, Abumoawad A, Woollard J, Lerman A, Textor S, Puranik A, Lerman L
    J Am Heart Assoc, 2019-06-01;8(11):e012584.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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