Human Prostasin/Prss8 Antibody Summary
Ala33-Gly319
Accession # Q16651
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Prostasin/Prss8 by Western Blot. Western blot shows lysates of human prostate tissue, LNCaP human prostate cancer cell line, PC-3 human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostasin/Prss8 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Prostasin/Prss8 in Human Prostate Cancer Tissue. Prostasin/Prss8 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 10 µg/mL Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Prostasin/Prss8 by Simple WesternTM. Simple Western lane view shows lysates of human prostate tissue and LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Prostasin/Prss8 at approximately 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Prostasin/Prss8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4599) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prostasin/Prss8
Prostasin, also known as channel activating protease 1, is a serine protease with trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of Prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of Prostasin is involved in the regulation of epithelial sodium channels (4, 5).
- Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
- Yu, J.X. et al. (1994) J. Biol. Chem. 269:18843.
- Yu, J.X. et al. (1995) J. Biol. Chem. 270:13483.
- Caldwell, R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C194.
- Tong, Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.
Product Datasheets
Citation for Human Prostasin/Prss8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin.
Authors: Brunati M, Perucca S, Han L et al.
Elife
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