Human Prostatic Acid Phosphatase/ACPP Antibody

Catalog #: MAB6240 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6240
MAB6240-SP

Detection of Human Prostatic Acid Phosphatase/ACPP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Prostatic Acid Phosphatase/ACPP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Prostatic Acid Phosphatase/ACPP in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TRACP/ACP5 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 690017

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Prostatic Acid Phosphatase/ACPP
Lys33-Gln379
Accession # P15309

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Prostatic Acid Phosphatase/ACPP by Western Blot. Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB6240) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostatic Acid Phosphatase/ACPP at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Prostatic Acid Phosphatase/ACPP in Human Prostate. Prostatic Acid Phosphatase/ACPP was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB6240) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hemotoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prostatic Acid Phosphatase/ACPP

ACPP (Acid phosphatase, prostate; also PAP and ACP3) is an approximately 50 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95‑100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature human ACPP is 354 amino acids (aa) in length (aa 33-386). It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, human ACPP shares 84% aa identity with mouse ACPP.

Entrez Gene IDs

55 (Human); 563181 (Mouse); 56780 (Rat)

Alternate Names

acid phosphatase, prostate; ACP3; ACP-3; ACPP; EC 3.1.3.2; PAP; Prostatic Acid Phosphatase; prostatic acid phosphotase