Human Protocadherin-1 Antibody

Catalog #: AF5899 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5899
AF5899-SP

Detection of Human Protocadherin-1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Protocadherin-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protocadherin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Protocadherin-8 and rhProtocadherin-10 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Protocadherin‑1
Thr58-Asn162
Accession # Q08174

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Protocadherin-1 by Western Blot. Western blot shows lysates of human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Human Protocadherin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5899) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Protocadherin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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Protocadherin-1 in BG01V Human Stem Cells. Protocadherin-1 was detected in immersion fixed BG01V human embryonic stem cells using Human Protocadherin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5899) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-1

Protocadherin-1 (PCDH-1; also PC42) is a 150 - 170 kDa δ1 subgroup member of the nonclustered protocadherin family of molecules. It is expressed on macrophages, respiratory epithelium, endothelial cells and neurons. Protocadherin-1 apparently forms homophilic Ca⁺⁺-dependent complexes and likely serves as an adhesion molecule. Human full-length Protocadherin-1 precursor is 1237 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 795 aa extracellular domain (ECD) (aa 58 - 852) plus a 364 aa cytoplasmic region. There are seven cadherin domains in the ECD (aa 58 - 844), and an RRVTF cytoplasmic motif that binds PP1 alpha phosphatase. There are multiple splice variants. Alternative start sites exist at Met23 and Met235, there is a deletion of aa 209 - 220, and a 27 aa substitution for aa 1034 - 1237. Over aa 58 - 162, human Protocadherin-1 shares 96% aa identity with mouse Protocadherin-1.

Entrez Gene IDs

5097 (Human); 75599 (Mouse); 307481 (Rat)

Alternate Names

cadherin-like 1; Cadherin-like protein 1; FLJ53887; MGC45991; PC42; PC42PCDH42; PCDH1; PCDH42; protocadherin 1 (cadherin-like 1); protocadherin 1; protocadherin 42; Protocadherin1; Protocadherin-1; protocadherin-42