Human Protocadherin-15 Antibody

Name: Human Protocadherin-15 Antibody
Brand: R&D Systems
SKU: MAB6729
Rating: 4.3 (3 reviews)

Catalog #: MAB6729 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6729
MAB6729-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Protocadherin-15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protocadherin-15 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 740523

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin-15
Tyr28-Ala1376
Accession # Q96QU1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Protocadherin‑15 in YT Human Cell Line. Protocadherin-15 was detected in immersion fixed YT human leukemia natural killer-like cell line using Mouse Anti-Human Protocadherin-15 Monoclonal Antibody (Catalog # MAB6729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-15

Protocadherin-15 (PCDH-15) is a 250-270 kDa "other" member of the nonclustered group of the protocadherin family of molecules. In the adult, it is expressed on the base of hair cell stereocilia in the Organ of Corti, on cerebellar granule cells, and on rod and cone photoreceptors. In the ear, it is expressed as a homodimer, and binds (in trans) to cadherin 23 homodimers on adjacent stereocilia. Mature human PCDH-15 is a type I transmembrane protein that is 1929 amino acids (aa) in length. It contains a 1350 aa extracellular domain (ECD) (aa 27-1376) plus a 558 aa cytoplasmic region. There are 11 cadherin domains in the ECD (aa 40-1259). Multiple splice forms are reported. One is a soluble 110 kDa form that shows a five aa substitution for aa 957-1955. Another contains a seven aa substitution for aa 1119-1125. Others show substitutions or deletions in either the extracellular or cytoplasmic domains. Over aa 27-1376 (the ECD), human PCDH-15 shares 95% aa sequence identity with mouse PCDH-15.

Entrez Gene IDs

65217 (Human); 11994 (Mouse); 690865 (Rat)

Alternate Names

autosomal recessive 23; cadherin-related family member 15; CDHR15; DFNB23; DKFZp667A1711; PCDH15; Protocadherin15; Protocadherin-15; protocadherin-related 15; USH1F