Human Protocadherin-19 Antibody

Catalog #: MAB8626 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8626
MAB8626-SP

Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Protocadherin-19 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protocadherin-19 in direct ELISA and flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 921614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Protocadherin-19
Met1-Ser678
Accession # Q8TAB3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

SH‑SY5Y human neuroblastoma cells and Jurkat cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Protocadherin-19 Monoclonal Antibody (Catalog # MAB8626, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Flow Cytometry

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Detection of Protocadherin-19 in Jurkat cells by Flow Cytometry. Jurkat cells were stained with Mouse Anti-Human Protocadherin-19 Monoclonal Antibody (Catalog # MAB8626, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-19

Protocadherin 19 (PCDH19) is a d2-group member of the of the protocadherin subfamily, cadherin family of molecules. PCDH19 is thought to be a calcium-dependent protein primarily expressed in the brain. The protein is also known as EFMR (Epilepsy Female-restricted with Mental Retardation).

Entrez Gene IDs

57526 (Human); 279653 (Mouse)

Alternate Names

EFMR; EFMR;EIEE9;KIAA1313;PCDH19; EIEE9; PCDH19; Protocadherin19; Protocadherin-19