Human Protocadherin-19 APC-conjugated Antibody

Catalog #: FAB8626A Datasheet / COA / SDS

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FAB8626A-025

Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry.

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Human Protocadherin-19 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Protocadherin-19 in direct ELISA and flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 921614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Protocadherin-19
Met1-Ser678
Accession # Q8TAB3

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

SH‑SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Protocadherin-19 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Protocadherin-19 APC-conjugated Monoclonal Antibody (Catalog # FAB8626A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Protocadherin-19 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human T cell leukemia cells were stained with Mouse Anti-Human Protocadherin-19 APC-conjugated Monoclonal Antibody (Catalog # FAB8626A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Protocadherin-19

Protocadherin 19 (PCDH19) is a member of the d2 subfamily of the non-(gene) clustered group of the PDCH (Protocadherin) family that belongs to the Cadherin superfamily of molecules. d2 subfamily members are characterized by both the absence of a Protein Phosphatase-1 alpha binding domain, and the presence of the two short amino acid motifs in their cytoplasmic domains. PDCH19 is found in the basal ganglia and hippocampus, and will undergo weak homophilic interaction. PDCH19 mutations have been associated with epilepsy and mental retardation.

Entrez Gene IDs

57526 (Human); 279653 (Mouse)

Alternate Names

EFMR; EFMR;EIEE9;KIAA1313;PCDH19; EIEE9; PCDH19; Protocadherin19; Protocadherin-19