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Human PSGR Antibody

Catalog #: MAB10669 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10669-100
MAB10669-SP
PSGR in Human Prostate.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PSGR Antibody Summary

Species Reactivity
Human
Specificity
Detects human PSGR in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1033009
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human PSGR synthetic peptide
Accession # Q9H255
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human normal prostate

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

PSGR in Human Prostate. PSGR was detected in immersion fixed paraffin-embedded sections of human normal prostate using Mouse Anti-Human PSGR Monoclonal Antibody (Catalog # MAB10669) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSGR

PSGR (OR51E2) is an olfactory receptor and part of a large family of GPCR's arising from single coding-exon genes.  Odorant receptors represent the largest GPCR family in the human genome and are found to be ectopically expressed in non-sensory tissues mediating a variety of cellular functions.  OR51E2 is well-characterized in prostate cancer and epidermal pigment cells and expressed in the cytosol and in the plasma membrane.  OR51E2 is involved in the regulation of RPE cell growth and is a potential target for the treatment of proliferative disorders.

Long Name
Prostate-Specific G-Protein Coupled Receptor
Entrez Gene IDs
81285 (Human)
Alternate Names
HPRAJ; Olfactory Receptor OR11-16; olfactory receptor, family 51, subfamily E, member 2; olfactory receptor, family 51, subfamily E, member 3 pseudogene; olfactory receptor, family 52, subfamily A, member 2; OR51E2; OR51E3P; OR52A2; prostate specific G-protein coupled receptor; Prostate-specific G-protein coupled receptor; PSGR; PSGRolfactory receptor 51E2

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Human PSGR Antibody
By Anonymous on 03/24/2022
Application: IHC Sample Tested: Prostate tissue Species: Human
Immunohistochemistry Human PSGR Antibody MAB10669