Human PTF1A Antibody

Name: Human PTF1A Antibody
Brand: R&D Systems
SKU: AF6119

Catalog #: AF6119 Datasheet / COA / SDS

Discontinued Product

AF6119 has been discontinued.

View all PTF1A products.

Detection of Human PTF1A by Western Blot.

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All PTF1A Antibodies

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Human PTF1A Antibody Summary

Species Reactivity

Human

Specificity

Detects human PTF1A in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PTF1A
Met1-Pro92
Accession # Q7RTS3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PTF1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PTF1A at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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PTF1A in Human Pancreatic Cancer Cells. PTF1A was detected in immersion fixed human pancreatic cancer cells using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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PTF1A in Human Pancreas. PTF1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTF1A

PTF1A (Pancreas transcription factor 1a; also p48 and bHLHA29) is a cell specific member of the bHLH family of proteins. Although its predicted MW is 41 kDa, a high ratio of Pro, Leu and Ala increases its MW to 48 kDa in SDS-PAGE. PTF1A forms a trimeric transcription factor complex with a p75 subunit (HEB, E2 or E47) and a p64 subunit (usually RBP-L). Within this complex, unfortunately termed PTF1, p48/PTF1A binds to a DNA E-box while p64 binds to a TC-box. This regulates the gene transcription of about 20 enzymes synthesized by pancreatic acinar cells. Human PTF1A is 328 amino acids (aa) in length. It contains one basic DNA binding HLH motif (aa 150-175) and a dimerization interface (aa 178-219). Over aa 1-92, human PTF1A shares 88% aa identity with mouse PTF1A.

Long Name

Pancreas-specific Transcription Factor 1a

Entrez Gene IDs

256297 (Human); 19213 (Mouse); 117034 (Rat)

Alternate Names

bHLH transcription factor p48; BHLHA29; bHLHa29exocrine pancreas-specific transcription factor p48; Class A basic helix-loop-helix protein 29; p48 DNA-binding subunit of transcription factor PTF1; pancreas specific transcription factor, 1a; pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; PTF1A; PTF1P48; PTF1-p48; PTF1-p48class II bHLH protein PTF1A