Human PTGER3 Antibody

Catalog #: MAB102431 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB102431-100
MAB102431-SP

Detection of Human PTGER3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PTGER3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PTGER3 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1026603

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human PTGER3 synthetic peptide
Accession # P43115

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HDLM‑2 human Hodgkin's lymphoma cell line and human kidney tissue

Immunohistochemistry

0.1-25 µg/mL

Immersion fixed paraffin-embedded sections of human kidney tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PTGER3 by Western Blot. Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB102431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PTGER3 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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PTGER3 in Human Kidney Tissue. PTGER3 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB102431) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGER3

Human Prostaglandin EP3 receptor, also known as EP3 or PTGER3, is a 53kDa prostaglandin receptor for prostaglandin E2 (PGE2) encoded by the PTGER3 gene. PTGER3 is a G protein-coupled receptor (GPCR) of the rhodopsin-like receptor family. Along with several other receptor subtypes (EP1, EP2, and EP4), it mediates the diverse biological effects of Prostaglandin E2 (PGE2). PGE2 has been implicated in numerous processes, including immune response modulation, male erectile function, induction of labor, cervical cancer, control of blood pressure, and asthma. Various isoforms are produced by alternative splicing. Prostaglandin E2 Receptor EP3 expression has been documented throughout the periphery, especially kidney. ESTs have been isolated primarily from kidney libraries.

Long Name

Prostaglandin E2 Receptor 3, Subtype EP3

Entrez Gene IDs

5733 (Human); 19218 (Mouse); 24929 (Rat)

Alternate Names

EP3; EP3EP3e; EP3-I; EP3-II; EP3-III; EP3-IV; MGC141828; MGC141829; MGC27302; PGE receptor EP3 subtype; PGE receptor, EP3 subtype; PGE2 receptor EP3 subtype; PGE2-R; prostaglandin E receotor EP3 subtype 3 isoform; prostaglandin E receptor 3 (subtype EP3); prostaglandin E2 receptor EP3 subtype; prostaglandin receptor (PGE-2); Prostanoid EP3 receptor; PTGER3