Human PXR/NR1I2 Antibody

Catalog #: PP-H4417-00
2 Citations Datasheet / COA / SDS

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PP-H4417-00

All PXR/NR1I2 Antibodies

Product Details

Citations (2)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PXR/NR1I2 Antibody Summary

Species Reactivity

Human

Specificity

This antibody specifically recognizes human PXR1 and human PXR2. Not yet tested in other species.

Source

Monoclonal Mouse IgG_2A Clone # H4417

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human PXR/NR1I2

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

This antibody can be used at 1 μg/mL under reducingconditions and 3 μg/mL under non-reducing conditions with the appropriatesecondary reagents to detect human PXR.

Immunoprecipitation

Optimal dilutions should be determined by eachlaboratory.

Direct ELISA

This antibody can be used at 2 μg/mL with the appropriatesecondary reagents to detect human PXR.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when stored at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: PXR/NR1I2

Pregnane X Receptor (PXR; also known as SXR and NR1I2) is a 50 kDa member of the Nuclear Receptor (NR1) subfamily of the Nuclear Hormone Receptor family. It is a product of the PXR gene that gives rise to two basic isoforms; a 434 amino acid (aa) PXR1 form and 473 aa PXR2 form. PXR2 differs from PXR1 in that PXR2 contains a 39 aa N-terminal extension. Six PXR1 isoforms have been reported (Swiss Protein Accession # 075469). The anti-human PXR antibody recognizes all isoforms except the "B" isoforms which show a deletion of amino acids 1 - 55 (MEVRP...FNVM). PXR forms a heterodimer with Retinoid X Receptor (RXR) and binds pregnanolone.

Long Name

Pregnane X Receptor

Entrez Gene IDs

8856 (Human); 18171 (Mouse)

Alternate Names

BXR; NR1I2; nuclear receptor subfamily 1 group I member 2; nuclear receptor subfamily 1, group I, member 2; ONR1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; PAR; PAR1; PAR2; Pregnane X receptor; PRR; PXR; PXRPARq; SAR; Steroid and xenobiotic receptor; SXR; SXRBXR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Steroid Hormone Receptors and Regulators

Citations for Human PXR/NR1I2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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