Human Pygopus-2 Antibody Summary
Met160-Glu336
Accession # Q9BRQ0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Pygopus‑2 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pygopus-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Pygopus-2 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Pygopus‑2 in Human Ovary and Human Ovarian Cancer Tissue. Pygopus-2 was detected in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel) using Goat Anti-Human Pygopus-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pygopus-2
Pygopus-2 is a 41 kDa nuclear protein that functions as a transcriptional co-activator in the Wnt signaling pathway. Human Pygopus-2 is synthesized as a 406 amino acid (aa) residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) zinc finger. The PHD zinc finger binds Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formulation of the quaternary complex is required for the activation of Wnt-responsive genes. Over the region used for immunization, human Pygopus-2 shares 95% amino acid sequence identity with the mouse protein.
Product Datasheets
Citation for Human Pygopus-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Pygo2 activates MDR1 expression and mediates chemoresistance in breast cancer via the Wnt/ beta -catenin pathway
Authors: Z-M Zhang, J-F Wu, Q-C Luo, Q-F Liu, Q-W Wu, G-D Ye et al.
Oncogene
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