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Human Pygopus-2 Biotinylated Antibody

Catalog #: BAF3616
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BAF3616
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Citations (1)
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Human Pygopus-2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pygopus-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Pygopus-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # Q9BRQ0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Pygopus‑2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pygopus-2

Pygopus-2 is a 41 kDa nuclear protein that functions as a transcriptional co-activator in the Wnt signaling pathway. Human Pygopus-2 is synthesized as a 406 amino acid (aa) residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) zinc finger. The PHD zinc finger binds Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formulation of the quaternary complex is required for the activation of Wnt-responsive genes. Over the region used for immunization, human Pygopus-2 shares 95% amino acid sequence identity with the mouse protein.

Entrez Gene IDs
90780 (Human); 68911 (Mouse)
Alternate Names
1190004M21Rik; FLJ33226; PYGO2; pygopus 2; pygopus homolog 2 (Drosophila); pygopus homolog 2; Pygopus2; Pygopus-2

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Citation for Human Pygopus-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Nuclear regulator Pygo2 controls spermiogenesis and histone H3 acetylation.
    Authors: Nair M, Nagamori I, Sun P, Mishra DP, Rheaume C, Li B, Sassone-Corsi P, Dai X
    Dev. Biol., 2008-06-06;320(2):446-55.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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