Human PYK2/FAK2 Biotinylated Antibody

Catalog #: BAF4589 Datasheet / COA / SDS

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BAF4589

Detection of Human PYK2/FAK2 by Western Blot.

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Human PYK2/FAK2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PYK2/FAK2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PYK2/FAK2
Asn221-Arg411
Accession # Q14289

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PYK2/FAK2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PYK2/FAK2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PYK2/FAK2 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PYK2/FAK2

PYK2 (Proline-rich tyrosine kinase 2; also FAK2, RAFTK and CAK beta ) is a 112‑116 kDa member of the FAK subfamily, tyrosine protein kinase family. It is expressed in multiple cell types, including endothelial cells, vascular smooth muscle cells, megakaryocytes and neurons. PYK2 is activated by elevated intracellular Ca⁺⁺ and is associated with MAPK pathway activation. Human PYK2 is 1009 amino acids (aa) in length. It contains one FERM domain that binds to growth factor receptors (aa 39‑359), a protein kinase domain (aa 425‑683), two Pro‑rich segments that bind SH domains (aa 702‑767 and 831‑869), and one FAT region that interacts with integrins (aa 868‑1009). Phosphorylation of PYK2 at Tyr402/579/580 is associated with changes in activity. There is one splice variant that shows a deletion of aa 739‑780.

Long Name

Proline-rich Tyrosine Kinase 2

Entrez Gene IDs

2185 (Human); 19229 (Mouse); 50646 (Rat)

Alternate Names

CADTK; CADTKCAK-beta; CAK beta; CAKB; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; EC 2.7.10; FADK2; FAK2; FAK2EC 2.7.10.2; Focal adhesion kinase 2; FRNK; PKB; ppyk2; Proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; protein-tyrosine kinase 2-beta; PTK; PTK2B protein tyrosine kinase 2 beta; PTK2B; PYK2; PYK2Related adhesion focal tyrosine kinase; RAFTK; RAFTKFADK 2