Human Rad50 Antibody Summary
Leu518-Leu881
Accession # Q92878
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Rad50 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cells and MCF‑7 human breast cancer cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Rad50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4996) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Rad50 at approximately ~150kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rad50
Rad50 is a 150 kDa member of the Rad50 subfamily, SMC (structural maintenance of chromosomes) family of DNA-associated genes. It is ubiquitously expressed, and associates with MRE11 and NBS1 to form an MRN complex. This complex stabilizes ATM kinase, thus contributing to DNA repair, and also participates in the suppression of DNA rereplication in dividing cells. Human Rad50 is 1312 amino acids (aa) in length. It has an apparent ATP binding site (aa 36‑43) plus a coiled‑coil region (aa 228‑598) followed by a “zinc-hook” domain (aa 635‑734) that mediates homodimerization. There are multiple splice variants. An alternate start site exists at Met140, there is a single Lys substitution for aa 723‑1312, and three Lys substitute for aa 555‑1312. Over aa 518‑881, human Rad50 is 96% aa identical to mouse Rad50.
Product Datasheets
Citation for Human Rad50 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Histone deacetylase inhibitor induces DNA damage, which normal but not transformed cells can repair.
Authors: Lee JH, Choy ML, Ngo L
Proc. Natl. Acad. Sci. U.S.A., 2010-08-02;107(33):14639-44.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Rad50 Antibody
There are currently no reviews for this product. Be the first to review Human Rad50 Antibody and earn rewards!
Have you used Human Rad50 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
