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Human RalB Antibody

Catalog #: MAB3920
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3920
MAB3920-SP

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Detection of Human RalB by Western Blot.
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Citations (2)
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Human RalB Antibody Summary

Species Reactivity
Human
Specificity
Detects human, mouse, and rat RalB in Western blots. In Western blots, no cross-reactivity with recombinant human RalA is observed.
Source
Monoclonal Rat IgG2A Clone # 399417
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human RalB
Met1-Leu206
Accession # P11234
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RalB antibody by Western Blot. View Larger

Detection of Human RalB by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human RalB Monoclonal Antibody (Catalog # MAB3920) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (10 ng/lane) were included. A specific band for RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RalB

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name
Ras-like Protein B
Entrez Gene IDs
5899 (Human); 64143 (Mouse); 116546 (Rat)
Alternate Names
GTP binding protein; RalB; RAS-like protein B; ras-related protein Ral-B; v-ral simian leukemia viral oncogene homolog B (ras related; GTP bindingprotein)

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Citations for Human RalB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Ral GTPases in Schwann cells promote radial axonal sorting in the peripheral nervous system
    Authors: Andrea Ommer, Gianluca Figlia, Jorge A. Pereira, Anna Lena Datwyler, Joanne Gerber, Jonathan DeGeer et al.
    Journal of Cell Biology
  2. Transcriptional signatures of Ral GTPase are associated with aggressive clinicopathologic characteristics in human cancer.
    Authors: Smith, Steven C, Baras, Alexande, Owens, Charles, Dancik, Garrett, Theodorescu, Dan
    Cancer Res, 2012-05-14;72(14):3480-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P

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