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Human RANK/TNFRSF11A PE-conjugated Antibody

Catalog #: FAB683P
3 Citations Datasheet / COA / SDS

Discontinued Product

FAB683P has been discontinued.
View all RANK/TNFRSF11A products.
Detection of RANK/TNFRSF11A in A431 Human Cell Line by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RANK/TNFRSF11A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human RANK/TNFRSF11A in ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymphotoxin beta R, rhNGF R, rhOPG, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse (rm) OX40, and rmRANK is observed.
Source
Monoclonal Mouse IgG1 Clone # 80704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RANK/TNFRSF11A
extracellular domain
Accession # Q9Y6Q6
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of RANK/TNFRSF11A antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of RANK/TNFRSF11A in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human RANK/TNFRSF11A PE-conjugated Monoclonal Antibody (Catalog # FAB683P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR]) and TNFRSF11A is a member of the tumor necrosis factor receptor family. The full length human RANK cDNA encodes a type I transmembrane protein of 616 amino acids with a predicted 184 amino acid extracellular domain and a 383 amino acid cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains. RANK is widely expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References
  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
Long Name
Receptor Activator of NF-kappa-B
Entrez Gene IDs
8792 (Human); 21934 (Mouse)
Alternate Names
CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator

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Citations for Human RANK/TNFRSF11A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins
    Authors: Blümke, A;Ijeoma, E;Simon, J;Wellington, R;Purwaningrum, M;Doulatov, S;Leber, E;Scatena, M;Giachelli, CM;
    Research square
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes
    Authors: D Steller, A Scheibert, T Sturmheit, SG Hakim
    Sci Rep, 2020-10-08;10(1):16861.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL.
    Authors: Riegel A, Maurer T, Prior B, Stegmaier S, Heppert V, Wagner C, Hansch G
    Eur J Immunol, 2012-04-01;42(4):975-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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