Human/Rat FABP1/L-FABP Antibody

Catalog #: MAB29643 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29643-100
MAB29643-SP

Detection of Human and Rat FABP1/L‑FABP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat FABP1/L-FABP Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat FABP1/L-FABP in direct ELISAs and Western blots. In sandwich immunoassays, it is specific for human FABP1/L-FABP.

Source

Monoclonal Mouse IgG₁ Clone # 965608

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FABP1/L-FABP
Met1-Ile127
Accession # P07148

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

ELISA

This antibody functions as an ELISA capture antibody for human FABP1/L-FABP when paired with Mouse Anti-Human FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29642).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat FABP1/L‑FABP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, Hep3B human hepatocellular carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29643) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Rat FABP1/L‑FABP by Simple Western^TM. Simple Western lane view shows lysates of rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB29643). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Long Name

Fatty Acid-Binding Protein 1

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Alternate Names

FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein