Human/Rat Laminin gamma 1 Antibody Summary
Applications
Green, T.L. et al. (1992) J. Biol. Chem. 267:2014. This application was not tested by R&D Systems.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Laminin gamma 1 in LS180 Human Cell Line. Laminin gamma1 was detected in immersion fixed LS180 human colorectal adenocarcinoma cell line using Mouse Anti-Human/Rat Laminin gamma1 Monoclonal Antibody (Catalog # MAB2139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin gamma 1
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentation, cell shape and migration, and maintenance of tissue phenotypes and survivial (1). Laminin gamma 1, also known as Laminin B2, is the gamma subunit of Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin).
Product Datasheets
Citations for Human/Rat Laminin gamma 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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PLAC1 is essential for FGF7/FGFRIIIb-induced Akt-mediated cancer cell proliferation
Authors: DB Roldán, M Grimmler, C Hartmann, S Hubich-Rau, T Bei beta ert, C Paret, G Cagna, C Rohde, S Wöll, M Koslowski, Ö Türeci, U Sahin
Oncotarget, 2020-05-19;11(20):1862-1875.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells.
Authors: Green TL, Hunter DD, Chan W, Merlie JP, Sanes JR
J. Biol. Chem., 1992-01-25;267(3):2014-22.
Species: Rat
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot
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