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Human/Rat Neuritin Antibody

Catalog #: AF283
4 Citations Datasheet / COA / SDS

Discontinued Product

AF283 has been discontinued.
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Detection of Human Neuritin by Western Blot.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Neuritin Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human Neuritin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neuritin
Ala28-Gly114
Accession # Q9NPD7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed cultured rat embryonic hippocampal neurons and rat brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Neuritin antibody by Western Blot. View Larger

Detection of Human Neuritin by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuritin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF283) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neuritin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuritin

Neuritin is a 14 kDa GPI-linked molecule that is expressed on developing and differentiating neurons as well as on hepatocytes. Neuritin is upregulated in response to BDNF, NT-3, androgens, and hypoxia, and it promotes neurite extension and arborization. Mature human Neuritin shares 99% and 100% aa sequence identity with mouse and rat Neuritin, respectively.

Entrez Gene IDs
51299 (Human)
Alternate Names
dJ380B8.2; neuritin 1; Neuritin; NRN1; NRNMGC44811

Product Datasheets

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Citations for Human/Rat Neuritin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Exogenous neuritin treatment improves survivability and functions of Schwann cells with improved outgrowth of neurons in rat diabetic neuropathy
    Authors: C Xi, Y Zhang, M Yan, Q Lv, H Lu, J Zhou, Y Wang, J Li
    J. Cell. Mol. Med., 2020-07-15;0(0):.
    Species: Rat
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  2. Neuritin Upregulates Kv4.2 ?-subunit of Potassium Channel Expression and Affects Neuronal Excitability by Regulating the Calcium-Calcineurin-NFATc4 Signaling Pathway
    Authors: Jin-Jing Yao
    J Biol Chem, 2016-06-15;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Neuritin attenuates cognitive function impairments in tg2576 mouse model of Alzheimer's disease.
    Authors: Choi, Yoori, Lee, Kihwan, Ryu, Junghwa, Kim, Hyoun Ge, Jeong, A Young, Woo, Ran-Sook, Lee, Jun-Ho, Hyun, Jin Won, Hahn, Seokyung, Kim, Joung-Hu, Kim, Hye-Sun
    PLoS ONE, 2014-08-07;9(8):e104121.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Neuritin mediates nerve growth factor-induced axonal regeneration and is deficient in experimental diabetic neuropathy.
    Authors: Karamoysoyli E, Burnand RC, Tomlinson DR, Gardiner NJ
    Diabetes, 2007-10-01;57(1):181-9.
    Species: Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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