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Human/Rat P-Selectin/CD62P Antibody

Catalog #: AF137
10 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF137
AF137-SP
Detection of Human P‑Selectin/CD62P by Western Blot.
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Product Details
Citations (10)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat P-Selectin/CD62P Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat P-Selectin/CD62P in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse P-Selectin, and less than 1% cross-reactivity with recombinant human (rh) E-Selectin and rhL-Selectin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human P‑Selectin/CD62P
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
Human platelets
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized recombinant human P‑Selectin (10 μg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 μg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human P-Selectin/CD62P antibody by Western Blot. View Larger

Detection of Human P‑Selectin/CD62P by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for P-Selectin/ CD62P at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Rat P-Selectin/CD62P antibody by Western Blot. View Larger

Detection of Rat P‑Selectin/CD62P by Western Blot. Western blot shows lysates of rat platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for P-Selectin/CD62P at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human P‑Selectin/CD62P by Simple WesternTM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for P‑Selectin/CD62P at approximately 219 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Selectin/CD62P

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

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Citations for Human/Rat P-Selectin/CD62P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Complement inhibition can decrease the haemostatic response in a microvascular bleeding model at multiple levels
    Authors: Murielle Golomingi, Jessie Kohler, Christina Lamers, Richard B. Pouw, Daniel Ricklin, József Dobó et al.
    Front Immunol
  2. Sulfation Modulates the Targeting Properties of Hyaluronic Acid to P-Selectin and CD44
    Authors: Deep S. Bhattacharya, Denis Svechkarev, Aishwarya Bapat, Prathamesh Patil, Michael A. Hollingsworth, Aaron M. Mohs
    ACS Biomaterials Science & Engineering
  3. P-selectin-dependent leukocyte adhesion is governed by endolysosomal two-pore channel 2
    Authors: Goretzko, J;Pauels, I;Heitzig, N;Thomas, K;Kardell, M;Na beta, J;Krogsaeter, EK;Schloer, S;Spix, B;Linard Matos, AL;Leser, C;Wegner, T;Glorius, F;Bracher, F;Gerke, V;Rossaint, J;Grimm, C;Rescher, U;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression
    Authors: E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai
    Nature Communications, 2021-03-26;12(1):1912.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Clathrin-mediated post-fusion membrane retrieval influences the exocytic mode of endothelial Weibel-Palade bodies
    Authors: NL Stevenson, IJ White, JJ McCormack, C Robinson, DF Cutler, TD Nightingal
    J. Cell. Sci., 2017-07-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
    Authors: Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin B, Zarbock A, Gerke V, Rescher U
    Nat Commun, 2014-04-28;5(0):3738.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC, Neutralization
  7. G protein-coupled receptor kinase 2 moderates recruitment of THP-1 cells to the endothelium by limiting histamine-invoked Weibel-Palade body exocytosis.
    Authors: Stevenson N, Martin-Martin B, Freeman J, Kriston-Vizi J, Ketteler R, Cutler D
    J Thromb Haemost, 2014-02-01;12(2):261-272.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
    Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
    ALTEX, 2011-01-01;28(4):305-16.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Elevated platelet microparticle levels in nonvalvular atrial fibrillation: relationship to p-selectin and antithrombotic therapy.
    Authors: Choudhury A, Chung I, Blann AD, Lip GY
    Chest, 2007-03-01;131(3):809-15.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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Human/Rat P-Selectin/CD62P Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human