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Human/Rat TC21/R-Ras2 Antibody

Catalog #: AF3605
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3605
AF3605-SP
Detection of Human/Rat TC21/R‑Ras2 by Western Blot.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat TC21/R-Ras2 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat TC21/R-Ras2 in Western blots. Does not detect recombinant human R-Ras or M-Ras (R‑Ras3).
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TC21/R-Ras2
Met1-Phe204
Accession # P62070
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Rat TC21/R‑Ras2 antibody by Western Blot. View Larger

Detection of Human/Rat TC21/R‑Ras2 by Western Blot. Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat TC21/R-Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for TC21/R-Ras2 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TC21/R-Ras2 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

TC21/R‑Ras2 in Human Placenta. TC21/R-Ras2 was detected in immersion fixed paraffin-embedded sections of normal human placenta using Goat Anti-Human/Rat TC21/R-Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TC21/R-Ras2

Teratocarcinoma oncogene 21 (TC21), also known as R-Ras2, is a small GTP-binding protein of the Ras superfamily of GTPases. TC21 comprises an R-Ras subfamily with fellow members R-Ras and M-Ras (R-Ras3). Overexpression and activating mutations of TC21 have been reported in several tumor tissues and cell lines.

Long Name
Teratocarcinoma Oncogene TC21
Entrez Gene IDs
22800 (Human); 66922 (Mouse); 365355 (Rat)
Alternate Names
ras-related protein R-Ras2; related RAS viral (r-ras) oncogene homolog 2; RRAS2; TC21; TC21Ras-like protein TC21; Teratocarcinoma oncogene

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Citations for Human/Rat TC21/R-Ras2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells.
    Authors: Shannon W, Nicole B, Amanda P et al.
    Cell Commun Signal.
  2. R-Ras subfamily proteins elicit distinct physiologic effects and phosphoproteome alterations in neurofibromin-null MPNST cells.
    Authors: Shannon W, Nicole B, Amanda P et al.
    Cell Commun Signal.

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