Home / Yes / Human/Rat Yes Antibody

Human/Rat Yes Antibody

Catalog #: AF3205
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3205
AF3205-SP
Detection of Human/Rat Yes by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Yes Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat Yes.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Yes
Met1-Gly88
Accession # P07947.3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Rat Yes antibody by Western Blot. View Larger

Detection of Human/Rat Yes by Western Blot. Western blot shows lysates of L6 rat myoblast cell line, Rat-2 rat embryonic fibroblast cell line, MCF-7 human breast cancer cell line and, MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Yes antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

Yes in A431 Human Cell Line. Yes was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Yes

Yes is the cellular homolog of the oncogenic protein encoded by the Yamaguchi 73 and Esh avian sarcoma viruses. After Src, Yes is the most widely expressed member of the Src family of nonreceptor tyrosine kinases. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

Long Name
V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1
Entrez Gene IDs
7525 (Human); 22612 (Mouse); 24884 (Rat)
Alternate Names
cellular yes-1 protein; c-yes; EC 2.7.10; EC 2.7.10.2; HsT441; P61-YES; Proto-oncogene c-Yes; proto-oncogene tyrosine-protein kinase Yes; v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1; Yamaguchi sarcoma oncogene; Yes; YES1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human/Rat Yes Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Docking of Syk to Fc epsilon RI is enhanced by Lyn but limited in duration by SHIP1
    Authors: William K. Kanagy, Cédric Cleyrat, Mohamadreza Fazel, Shayna R. Lucero, Marcel P. Bruchez, Keith A. Lidke et al.
    Molecular Biology of the Cell

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Rat Yes Antibody

There are currently no reviews for this product. Be the first to review Human/Rat Yes Antibody and earn rewards!

Have you used Human/Rat Yes Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review