Human RBP4 Magnetic Luminex Performance Assay
Human RBP4 Magnetic Luminex Performance Assay Summary
| Assay Type | bead-based multiplex assay for the Luminex® platform |
| Format | 1 x 96-well microplate and antibody-coated beads |
| Analytes Detected | Please see analyte list in assay customization tool below. |
| Performance Validation | Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity. |
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Precision
|
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. |
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| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 67 | 65 | 67 |
| Mean (pg/mL) | 309 | 2116 | 13,990 | 288 | 1978 | 12,875 |
| Standard deviation | 18.7 | 35.5 | 555 | 44.5 | 174 | 1102 |
| CV (%) | 6.1 | 1.7 | 4.0 | 15.4 | 8.8 | 8.6 |
Linearity
|
Samples containing and/or spiked with human RBP4 were serially diluted to evaluate assay linearity. |
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| Serum | EDTA plasma | Heparin plasma | Urine | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 92 | 93 | 95 | 86 | ||||
| Range (%) | 85-100 | 89-96 | 92-101 | 83-92 | |||||
| 1:4 | Average % of Expected | 97 | 97 | 97 | 99 | ||||
| Range (%) | 96-100 | 95-99 | 95-98 | 98-101 | |||||
| 1:8 | Average % of Expected | 99 | 98 | 94 | 103 | ||||
| Range (%) | 96-103 | 96-103 | 93-94 | 102-104 | |||||
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Background: RBP4/Retinol-Binding Protein 4
Retinol-binding protein 4 (RBP4) is a Lipocalin superfamily molecule that transports vitamin A (retinol) and retinaldehyde in the serum. Retinaldehyde is the critical chromophore in the rhodopsin photoreceptor, while both it and retinoic acid regulate a multitude of cellular differentiation and proliferation effects through the intracellular receptors RAR and RXR. RBP4 is secreted primarily by hepatocytes and adipocytes into the blood where the RBP4-retinol complex interacts with transthyretin (TTR). Formation of the complex with TTR increases the serum half-life of RBP4 by preventing RBP4 filtration through the kidney. The C-terminally processed forms of RBP4, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. RBP4 promotes hyperglycemia, and its upregulation in visceral and liver adipocytes leads to elevated serum levels.
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