Human RECK Antibody

Catalog # Availability Size / Price Qty
MAB1734
MAB1734-SP
Product Details
Citations (1)
FAQs
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Human RECK Antibody Summary

Species Reactivity
Human
Specificity
Detects human RECK in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 198212
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RECK
Ala29-Val934
Accession # O95980
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human RECK under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human RECK, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RECK

RECK is a GPI-anchored glycoprotein expressed in many normal tissues and down-regulated in many tumors and cells transformed by various oncogenes. It acts via a post-transcriptional event to decrease secretion of MMP-9.

Long Name
Reversion-inducing-cysteine-rich Protein with Kazal Motifs
Entrez Gene IDs
8434 (Human); 53614 (Mouse)
Alternate Names
hRECK; membrane-anchored glycoprotein (metastasis and invasion); RECK; reversion-inducing-cysteine-rich protein with kazal motifs; ST15; ST15reversion-inducing cysteine-rich protein with Kazal motifs; suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein withkazal motifs); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein withkazal motifs); Suppressor of tumorigenicity 15 protein

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Citation for Human RECK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of histone deacetylase attenuates hypoxia-induced migration and invasion of cancer cells via the restoration of RECK expression.
    Authors: Jeon HW, Lee YM
    Mol. Cancer Ther., 2010-05-04;9(5):1361-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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