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Human RECK Antibody

Catalog #: AF1734
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1734
AF1734-SP
Detection of Human RECK by Western Blot.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RECK Antibody Summary

Species Reactivity
Human
Specificity
Detects human RECK in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RECK
Gly27-Val934
Accession # O95980
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human RECK, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RECK antibody by Western Blot. View Larger

Detection of Human RECK by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RECK at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RECK

RECK is a GPI-anchored membrane protein that is highly expressed in most of the human tissues and untransformed cells, but is undetectable in tumor cells. RECK can negatively regulate MMP-9, MMP-2 and MTI-MMP activity and inhibit tumor angiogenesis and metastasis.

Long Name
Reversion-inducing-cysteine-rich Protein with Kazal Motifs
Entrez Gene IDs
8434 (Human); 53614 (Mouse)
Alternate Names
hRECK; membrane-anchored glycoprotein (metastasis and invasion); RECK; reversion-inducing-cysteine-rich protein with kazal motifs; ST15; ST15reversion-inducing cysteine-rich protein with Kazal motifs; suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein withkazal motifs); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein withkazal motifs); Suppressor of tumorigenicity 15 protein

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Citations for Human RECK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Effects of CoCl2-simulated hypoxia on the expression levels of matrix metalloproteinases in renal adenocarcinoma cells and renal tubular epithelial cells
    Authors: Zhang X, Chen L.
    Exp Ther Med
  2. RECK controls breast cancer metastasis by modulating a convergent, STAT3-dependent neoangiogenic switch.
    Authors: Walsh, L A, Roy, D M, Reyngold, M, Giri, D, Snyder, A, Turcan, S, Badwe, C R, Lyman, J, Bromberg, J, King, T A, Chan, T A
    Oncogene, 2014-06-16;34(17):2189-203.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  3. MT1-MMP and RECK are involved in human CD34+ progenitor cell retention, egress, and mobilization.
    Authors: Vagima Y, Avigdor A, Goichberg P, Shivtiel S, Tesio M, Kalinkovich A, Golan K, Dar A, Kollet O, Petit I, Perl O, Rosenthal E, Resnick I, Hardan I, Gellman YN, Naor D, Nagler A, Lapidot T
    J. Clin. Invest., 2009-02-09;119(3):492-503.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. miR-21 plays a pivotal role in gastric cancer pathogenesis and progression.
    Authors: Zhang Z, Li Z, Gao C, Chen P, Chen J, Liu W, Xiao S, Lu H
    Lab. Invest., 2008-09-15;88(12):1358-66.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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