Human Renin Antibody Summary
Leu24-Arg406
Accession # P00797
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Renin Monoclonal Antibody (Catalog # MAB4090).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Renin DuoSet ELISA Kit (Catalog # DY4090) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Human Renin ELISA Standard Curve. Recombinant Human Renin protein was serially diluted 2-fold and captured by Mouse Anti-Human Renin Monoclonal Antibody (Catalog # MAB4090) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Renin Monoclonal Antibody (Catalog # MAB40901) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Renin
Human Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney (1). Renin differs from the other members of this class by having a pH optimum near the neutral pH region with native substrates instead of a pH 2.0 to 3.4 range (2). This more neutral pH optimum allows it to be functional in the plasma. Renin also has a very high selectivity for substrates due to a long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by Renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. Renin releases the decapeptide angiotensin I, which in turn is further converted to vasoactive hormone angiotensin II by angiotensin converting enzyme (ACE). Renin is produced as prorenin with 43 pro residues at the N‑terminal of mature Renin. The inactive prorenin becomes activated proteolytically by trypsin, cathepsin B, or other proteinases.
- Yokosawa, H. et al. (1980) J. Biol. Chem. 255:3498.
- Fuminaki, S. et al. (2004) in Handbook of Proteolytic Enzymes, Barret, A. J. et al. eds. p. 54.
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