Human Rev-erb A alpha /NR1D1 Antibody

Catalog #: PP-A8740A-00 Datasheet / COA / SDS

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PP-A8740A-00

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Rev-erb A alpha /NR1D1 Antibody Summary

Species Reactivity

Human

Specificity

This antibody specifically recognizes human Rev-Erb alpha but does not recognize human Rev-Erb beta. Not yet tested in other species.

Source

Monoclonal Mouse IgG_2A Clone # A8740A

Purification

Protein A or G purified from ascites

Immunogen

Recombinant human Rev-Erb alpha /NR1D1
aa 2-130

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

This antibody can be used at 1 μg/mL under reducing and non-reducing conditions with the appropriate secondary reagents to detect human Rev-Erb alpha.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Direct ELISA

This antibody can be used at 10 ng/mL with the appropriatesecondary reagents to detect human Rev-Erb alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70° C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Rev-erb A alpha/NR1D1

Reverse orientation c-erbA-1 gene alpha (Rev-Erb alpha, ear1; NR1D1) is a member of the Orphan Nuclear Receptor family. Rev-Erb alpha is expressed in skeletal muscles, brown fat, liver, heart, brain, pituitary, kidney, testis, lung, and hypothalamus. Rev-Erb alpha has broad roles in cell proliferation and physiology. Rev-Erb alpha expression is regulated along a circadian rhythm.

Entrez Gene IDs

9572 (Human)

Alternate Names

Ear-1; EAR1nuclear receptor subfamily 1 group D member 1; HREV; NR1D1; nuclear receptor subfamily 1, group D, member 1; Reverb A alpha; Rev-erb A alpha; Rev-ErbAalpha; Rev-erbA-alpha; THRA1; THRAL; THRALrev-erbA-alpha; V-erbA-related protein 1