Human RIPK2/RIP2 Antibody

Catalog #: MAB103872 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB103872-100
MAB103872-SP

Detection of Human RIPK2/RIP2 by Western Blot.

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All RIPK2/RIP2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RIPK2/RIP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RIPK2/RIP2 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2527A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E.coli-derived recombinant human RIPK2/RIP2
Met1-Met540
Accession # O43353

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RIPK2/RIP2 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103872) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for RIPK2/RIP2 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RIPK2/RIP2

Receptor-interacting serine/threonine-protein kinase 2, also known as RIPK2 or RIP-2, is a 540 amino acids (aa) Serine/threonine/tyrosine kinase encoded by the RIPK2 gene. RIP-2, a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, plays an essential role in modulation of innate and adaptive immune responses. Human RIP2 is an adapter protein necessary for signal propagation of the Nucleotide-binding-oligomerization-domain-containing proteins 1/2 (NOD1 and NOD2). Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through the C-terminal caspase recruitment domain (CARD). Human and mouse RIPK2 aa sequences are 84% identical .

Long Name

Receptor Interacting Serine/Threonine Kinase 2

Entrez Gene IDs

8767 (Human); 192656 (Mouse)

Alternate Names

CARD3; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAKCCK; EC 2.7.11; EC 2.7.11.1; GIG30; growth-inhibiting gene 30; receptor interacting protein 2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase; Receptor-interacting protein 2; receptor-interacting serine/threonine-protein kinase 2; receptor-interacting serine-threonine kinase 2; RICK; RICKGIG30; RIP2; RIP-2; RIP2CARD-carrying kinase; RIPK2; RIP-like-interacting CLARP kinase