Human Rit1 Antibody

Catalog #: MAB4697 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4697
MAB4697-SP

Detection of Human Rit1 by Western Blot.

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Human Rit1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Rit1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 456201

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rit1
Met1-Thr219
Accession # Q92963

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Rit1 by Western Blot. Western blot shows lysates of human brain tissue and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rit1 Monoclonal Antibody (Catalog # MAB4697) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rit1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rit1

Rit1 is a member of the Ras-related small GTPase superfamily. The 219 amino acid, 25 kDa human Rit1 is a cytoplasmic cell membrane-associated protein that is expressed in most tissues. It is widely expressed in the developing and adult nervous systems and participates in NGF-induced axon outgrowth. Rit1 forms complexes with other small GTPases, such as cdc42 and Rac1, that are important during cell growth and polarity. Human Rit1 shares 95% amino acid identity with mouse and rat Rit1.

Long Name

Ras-like without CAAX 1

Entrez Gene IDs

6016 (Human); 19769 (Mouse); 499652 (Rat)

Alternate Names

GTP-binding protein Rit1; GTP-binding protein Roc1; MGC125864; MGC125865; Ras-like protein expressed in many tissues; Ras-like without CAAX 1; Ras-like without CAAX protein 1; RIBB; RIBBRIT; Ric-like, expressed in many tissues; RIT; Rit1; ROC1; ROC1Ric (Drosophila)-like, expressed in many tissues