Human Rit2 Antibody

Catalog #: MAB6094 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6094
MAB6094-SP

Detection of Human Rit2 by Western Blot.

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Human Rit2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Rit2 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 456313

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Rit2
aa 1-217
Accession # Q99578

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Immersion-fixed paraffin-embedded sections of human brain (caudate putamen)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Rit2 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human brain (hypothalamus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Rit2 Monoclonal Antibody (Catalog # MAB6094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rit2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rit2

GTP-binding protein Rit2 (Rit2; also Rin, Ras-like protein expressed in neurons, Ras-like without CAAX) is a 25 kDa member of the small GTPase superfamily and Ras family. Human Rit2 is 217 amino acids (aa) in length. Like all small G-proteins, Rit2 contains five highly conserved domains (G1-G5) and acts as a molecular switch by alternating an active GTP-bound form and an inactive GDP-bound form. There are two isoforms for Rit2. Isoform 1 is the long form. Isoform 2 has an 11 aa substitution for residues 143‑153 found in isoform 1 and a deletion of aa 154‑217. Rit2 is expressed exclusively in neurons. In addition, Rit2 binds calmodulin through a C‑terminal binding motif.

Long Name

Ras-like without CAAX 2

Entrez Gene IDs

6014 (Human); 19762 (Mouse); 291713 (Rat)

Alternate Names

GTP-binding protein Rit2; GTP-binding protein Roc2; Ras-like protein expressed in neurons; Ras-like without CAAX 2; Ras-like without CAAX protein 2; RIBA; Ric (Drosophila)-like, expressed in neurons; Ric-like, expressed in neurons; RIN; Rit2; ROC2