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Human ROBO1 Antibody

Catalog #: MAB71181
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB71181
MAB71181-SP
Detection of ROBO1 in HepG2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROBO1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROBO1 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 770502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO1
Met1-Ala858
Accession # Q9Y6N7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ROBO1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ROBO1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ROBO1 Monoclonal Antibody (Catalog # MAB71181, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry ROBO1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

ROBO1 in HepG2 Human Cell Line. ROBO1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human ROBO1 Monoclonal Antibody (Catalog # MAB71181) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO1

ROBO1 (Roundabout-like protein 1) is a 190-230 kDa member of the ROBO/roundabout receptor family. It is expressed by commissural axons from multiple nuclei, and is also found on vascular endothelium, bronchial epithelium, and synctiotrophoblasts. It interacts with Slit and DCC to temporally regulate the migration of axonal processes. The human ROBO1 precursor is a 1651 amino acid (aa) type I transmembrane protein. It contains a 25 aa signal sequence, followed by an 872 aa extracellular region (aa 26-897) that possesses five C2-type Ig-like domains (aa 68-541) and three fibronectin type III domains (aa 561-864) (SwissProt # Q9Y6N7). ROBO1 shows multiple isoform variants. The variant used here is termed ROBO1b/DUTT1 (Genbank # NP_598334), and it possesses an 18 aa substitution for aa 1‑47, accompanied by a three aa insertion after Gln348, and a deletion of aa 939-947. ROBO1a, by contrast, possesses only the 18 aa substitution just described. A third isoform possesses the same changes as ROBO1b plus an additional deletion of aa 1013-1067, while a final variant utilizes an alternative start site at Met120. Proteolytic cleavage generates a soluble 120 kDa N-terminal fragment. Over aa 20-861, human ROBO1b shares 97% aa identity with mouse ROBO1.

Long Name
Roundabout Homolog 1
Entrez Gene IDs
6091 (Human); 58946 (Rat)
Alternate Names
DUTT1; DUTT1Drosophila) homolog 1; H-Robo-1; MGC131599; MGC133277; ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila)

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Citations for Human ROBO1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The neurorepellent, Slit2, prevents macrophage lipid loading by inhibiting CD36-dependent binding and internalization of oxidized low-density lipoprotein
    Authors: B Yusuf, I Mukovozov, S Patel, YW Huang, GY Liu, EC Reddy, M Skrtic, M Glogauer, LA Robinson
    Scientific Reports, 2021-02-11;11(1):3614.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Lysates, ICC
  2. Slit2 Modulates the Inflammatory Phenotype of Orbit-Infiltrating Fibrocytes in Graves' Disease
    Authors: R Fernando, ABD Grisolia, Y Lu, S Atkins, TJ Smith
    J. Immunol., 2018-05-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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