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Human ROBO3 Antibody

Catalog #: AF3076
23 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3076
AF3076-SP
Product Details
Citations (23)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROBO3 Antibody Summary

Species Reactivity
Human
Specificity
Detects ROBO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross-reactivity with recombinant mouse ROBO3 is observed, 5% cross-reactivity with recombinant human ROBO2 and recombinant rat ROBO1 is observed, and less than 1% cross‑reactivity with recombinant human ROBO4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO3
Leu40-Ser545
Accession # Q96MS0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ROBO3 Fc Chimera (Catalog # 3076-RB)
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of embryonic mouse spinal cord (E11.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO3

Human ROBO3 is a 200 kDa member of the ROBO family of guidance molecules (1‑3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO3 is a type I transmembrane glycoprotein that is synthesized as a 1386 amino acid (aa) precursor. It contains a 20 aa signal sequence, an 871 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 474 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 64‑531) and three fibronectin (FN) type III domains (aa 555‑863). The cytoplasmic region contains three of four possible 15‑20 aa long CC (conserved cytoplasmic) motifs that are found in ROBO1 (7, 8). Human ROBO3 has multiple isoforms. An alternate start site generates a 1365 aa A isoform and a 1341 aa B isoform. These two mature forms differ only over the first 26 and 2 amino acids of the N-terminus, respectively. There are multiple point mutations and insertions in the ROBO3 gene. Three result in truncated forms. One is 456 aa in length and ends after the Ig-like domain # 4. A second is 770 aa in length and ends in the third FN domain. A third isoform is truncated after aa 1108 in the cytoplasmic region after CC2. At least one alternate splice form is also reported. It shows a 10 aa substitution between aa 1025‑1034, followed by truncation. Human ROBO3 ECD is 84% and 91% aa identical to the ECD in mouse and canine ROBO3, respectively. Normally, axons originating on one side of the spinal cord are inhibited from crossing to the other side by a SLIT2-ROBO1 interaction at the midline. ROBO3 is permissive for this event. It is unclear how this is accomplished. One possibility is that it binds directly to ROBO1, blocking SLIT activation. A second possibility involves ROBO3 binding to SLIT2 in a nonproductive interaction. However, only ROBO3 Form B is known to bind to SLIT2 (9‑11).

References
  1. Rajagopalan, S. et al. (2000) Neuron 28:767.
  2. Guthrie, S. (2004) Curr. Biol. 14:R632.
  3. Guthrie, S. (2001) Curr. Biol. 11:R300.
  4. Seeger, M. et al. (1993) Neuron 10:409.
  5. Jen, J.C. et al. (2004) Science 304:1509.
  6. Swiss-Prot. Accession # Q96MS0.
  7. Bashaw, G.J. et al. (2000) Cell 101:703.
  8. Kidd, T. et al. (1998) Cell 92:205.
  9. Camurri, L. et al. (2005) Mol. Cell. Neurosci. 30:485.
  10. Sabatier, C. et al. (2004) Cell 117:157.
  11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
Long Name
Roundabout Homolog 3
Entrez Gene IDs
64221 (Human); 19649 (Mouse)
Alternate Names
FLJ21044; HGPPS; HGPS; RBIG1horizontal gaze palsy with progressive scoliosis; retinoblastoma inhibiting gene 1; RIG1; ROBO3; roundabout (axon guidance receptor, Drosophila) homolog 3; roundabout homolog 3; roundabout, axon guidance receptor, homolog 3 (Drosophila); Roundabout-like protein 3

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Citations for Human ROBO3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Netrin-1 Derived from the Ventricular Zone, but not the Floor Plate, Directs Hindbrain Commissural Axons to the Ventral Midline
    Authors: Kenta Yamauchi, Maya Yamazaki, Manabu Abe, Kenji Sakimura, Heiko Lickert, Takahiko Kawasaki et al.
    Scientific Reports
  2. Neurogenesis changes beta -catenin shipping address from adherens junctions to nucleus to booster axonal growth
    Authors: Antonio Herrera, Anghara Menendez, Andrea Ochoa, Lídia Bardia, Julien Colombelli, Sebastian Pons
    Development
  3. VEGF Mediates Commissural Axon Chemoattraction through Its Receptor Flk1
    Authors: Carmen Ruiz de Almodovar, Pierre J. Fabre, Ellen Knevels, Cathy Coulon, Inmaculada Segura, Patrick C.G. Haddick et al.
    Neuron
  4. Netrin 1-Mediated Role of the Substantia Nigra Pars Compacta and Ventral Tegmental Area in the Guidance of the Medial Habenular Axons
    Authors: Verónica Company, Abraham Andreu-Cervera, M. Pilar Madrigal, Belén Andrés, Francisca Almagro-García, Alain Chédotal et al.
    Frontiers in Cell and Developmental Biology
  5. Robo2 Receptor Gates the Anatomical Divergence of Neurons Derived From a Common Precursor Origin
    Authors: Maud Wurmser, Mridula Muppavarapu, Christine Mary Tait, Christophe Laumonnerie, Luz María González-Castrillón, Sara Ivy Wilson
    Frontiers in Cell and Developmental Biology
  6. Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord
    Authors: SG Varadaraja, JH Kong, KD Phan, TJ Kao, SC Panaitof, J Cardin, H Eltzschig, A Kania, BG Novitch, SJ Butler
    Neuron, 2017-04-21;0(0):.
  7. Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion
    Authors: Karina Chaudhari, Madhavi Gorla, Chao Chang, Artur Kania, Greg J Bashaw
    eLife
  8. Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
    Authors: Sandeep Gupta, Ken Yamauchi, Bennett G. Novitch, Samantha J. Butler
    STAR Protocols
  9. Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control
    Authors: Schlienger, S;Yam, PT;Balekoglu, N;Ducuing, H;Michaud, JF;Makihara, S;Kramer, DK;Chen, B;Fasano, A;Berardelli, A;Hamdan, FF;Rouleau, GA;Srour, M;Charron, F;
    Science advances
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  10. Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs
    Authors: Y Zang, K Chaudhari, GJ Bashaw
    Cell Reports, 2022-12-06;41(10):111785.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  11. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
    Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
    Cell Rep, 2020-11-24;33(8):108425.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  12. Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation
    Authors: Y Zhu, T Hirata, F Mackay, F Murakami
    Sci Rep, 2020-07-16;10(1):11830.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection
    Authors: S Skarlatou, C Hérent, E Toscano, CS Mendes, J Bouvier, N Zampieri
    Cell Rep, 2020-06-09;31(10):107741.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies
    Authors: WW Feng, O Wilkins, S Bang, M Ung, J Li, J An, C Del Genio, K Canfield, J DiRenzo, W Wells, A Gaur, RB Robey, JY Guo, RL Powles, C Sotiriou, L Pusztai, M Febbraio, C Cheng, WB Kinlaw, M Kurokawa
    Cell Rep, 2019-12-10;29(11):3405-3420.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance
    Authors: J Ferent, S Constable, ED Gigante, PT Yam, LE Mariani, E Legué, KF Liem, T Caspary, F Charron
    Cell Rep, 2019-12-10;29(11):3356-3366.e3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
    Authors: M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw
    Cell Rep, 2019-03-19;26(12):3298-3312.e4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  17. The ciliopathy gene ftm/rpgrip1l controls mouse forebrain patterning via region-specific modulation of hedgehog/gli signaling
    Authors: A Andreu-Cer, I Anselme, A Karam, C Laclef, M Catala, S Schneider-
    J. Neurosci., 2019-01-28;0(0):.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  18. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance
    Authors: C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal
    Nature, 2017-04-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors.
    Authors: Samelson B, Gore B, Whiting J, Nygren P, Purkey A, Colledge M, Langeberg L, Dell'Acqua M, Zweifel L, Scott J
    J Biol Chem, 2015-04-16;290(22):14107-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. The chemorepellent Slit3 promotes monocyte migration.
    Authors: Geutskens SB, Hordijk PL, van Hennik PB
    J. Immunol., 2010-11-15;185(12):7691-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Non-cell autonomous control of precerebellar neuron migration by Slit and Robo proteins
    Authors: Chloé Dominici, Quentin Rappeneau, Pavol Zelina, Stéphane Fouquet, Alain Chédotal
    Development
  22. SlitC-PlexinA1 mediates iterative inhibition for orderly passage of spinal commissural axons through the floor plate
    Authors: Hugo Ducuing, Thibault Gardette, Aurora Pignata, Karine Kindbeiter, Muriel Bozon, Olivier Thoumine et al.
    eLife
  23. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing
    Authors: Berenice Hernandez-Enriquez, Zhuhao Wu, Edward Martinez, Olav Olsen, Zaven Kaprielian, Patricia F. Maness et al.
    Genes & Development

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