Human ROR alpha /NR1F1 Antibody Summary
Arg47-His163
Accession # P35398
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ROR alpha /NR1F1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P) and either (A) Mouse Anti-Human RORa/NR1F1 Monoclonal Antibody (Catalog # MAB8924) or (B) Mouse IgG1Isotype Control (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012).
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Detection of ROR alpha /NR1F1 in Jurkat cells by Flow Cytometry Jurkat cells were stained with Mouse Anti-Human ROR alpha /NR1F1 Monoclonal Antibody (Catalog # MAB8924, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ROR alpha/NR1F1
RORa (Retinoid-related Orphan Receptor alpha ) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression. Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha.
Product Datasheets
Citation for Human ROR alpha /NR1F1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Integrative transcriptome and chromatin landscape analysis reveals distinct epigenetic regulations in human memory B cells
Authors: JB Moroney, A Vasudev, A Pertsemlid, H Zan, P Casali
Nat Commun, 2020-10-28;11(1):5435.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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