Human ROR gamma t/RORC2/NR1F3 Antibody

Catalog #: MAB61093 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB61093-100
MAB61093-SP

Detection of ROR gamma /RORC/NR1F3 in Human PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ROR gamma t/RORC2/NR1F3 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1181A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E.coli derived recombinant human ROR gamma T/RORC2/NR1F3
Met1-Gln100
Accession # P51449

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012) and HepG2 cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of ROR?/RORC/NR1F3 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of ROR gamma /RORC/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human ROR gamma/RORC/NR1F3 Monoclonal Antibody (Catalog # MAB61093) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

View Larger

Detection of ROR gamma t/RORC2/NR1F3 in HepG2 cells by Flow Cytometry. HepG2 cells were stained with Rabbit Anti-Human ROR gamma t/RORC2/NR1F3 Monoclonal Antibody (Catalog # MAB61093, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name

Retinoic Acid-Related Orphan Receptor gamma t

Entrez Gene IDs

6097 (Human)

Alternate Names

IMD42;NR1F3;Nuclear receptor ROR-gamma;RORG;RZRG;RZR-GAMMA;TOR; ROR gamma t; RORC2