Name: Human RUNX1/CBFA2 Antibody
Brand: R&D Systems
SKU: MAB23991
Price: 429 USD

Species Reactivity

Human

Specificity

Detects human RUNX1/CBFA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) RUNX2 or rhRUNX3 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 751926

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human RUNX1/CBFA2
Gly217-Tyr480
Accession # Q01196-8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RUNX1/CBFA2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB23991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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RUNX1/CBFA2 in K562 Human Cell Line. RUNX1/CBFA2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human RUNX1/CBFA2 Monoclonal Antibody (Catalog # MAB23991) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot

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Detection of Porcine RUNX1/CBFA2 by Western Blot RUNX1 enhances TGEV-induced apoptosis.(A) Silencing effect of RUNX1 siRNAs on RUNX1 at mRNA level. PK-15 cells were transfected with RUNX1-specific siRNA or irrelevant siRNA and measured by real-time PCR (normalized to beta -actin). (B) The silencing effect of siRUNX1-2 on RUNX1 expression. (C) The effect of siRUNX1-2 on PK-15-cell viability. The cells were incubated after transfecting with 100 nM siRUNX1-2 for 48 h. Cell viability was evaluated by CCK-8 assay. (D) The over-expression of RUNX1 using pCI-neo-RUNX1. PK-15 cells were transfected with pCI-neo- RUNX1 or pCI-neo vector, and the expression level of RUNX1 was assessed by western blot at 48 hpt. (E) The effect of RUNX1 on the expression of Bax. (F) The effect of RUNX1 on caspase-9 activity. (G) The effect of RUNX1 on caspase-3 activity. Data represent mean ± S.D. of three independent experiments. ∗P < 0.05 in comparison with the control. ∗∗P < 0.01 in comparison with the control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26870610), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot

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Detection of Porcine RUNX1/CBFA2 by Western Blot RUNX1 enhances TGEV-induced apoptosis.(A) Silencing effect of RUNX1 siRNAs on RUNX1 at mRNA level. PK-15 cells were transfected with RUNX1-specific siRNA or irrelevant siRNA and measured by real-time PCR (normalized to beta -actin). (B) The silencing effect of siRUNX1-2 on RUNX1 expression. (C) The effect of siRUNX1-2 on PK-15-cell viability. The cells were incubated after transfecting with 100 nM siRUNX1-2 for 48 h. Cell viability was evaluated by CCK-8 assay. (D) The over-expression of RUNX1 using pCI-neo-RUNX1. PK-15 cells were transfected with pCI-neo- RUNX1 or pCI-neo vector, and the expression level of RUNX1 was assessed by western blot at 48 hpt. (E) The effect of RUNX1 on the expression of Bax. (F) The effect of RUNX1 on caspase-9 activity. (G) The effect of RUNX1 on caspase-3 activity. Data represent mean ± S.D. of three independent experiments. ∗P < 0.05 in comparison with the control. ∗∗P < 0.01 in comparison with the control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26870610), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

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Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX1/CBFA2

RUNX1 (runt-related transcription factor 1), also known as CBFA2 (core-binding factor alpha 2) or AML1 (acute myelogenous leukemia 1), is a heterodimeric transcription factor that plays a role in normal hematopoiesis. Multiple splice variants exist. Defects in RUNX1 are associated with several types of leukemia.

Long Name

Runt-related Transcription Factor 1

Entrez Gene IDs

861 (Human); 12394 (Mouse); 50662 (Rat)

Alternate Names

Acute myeloid leukemia 1 protein; AML1; AML1acute myeloid leukemia 1; AML1-EVI-1; CBFA2; CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1; Core-binding factor subunit alpha-2; EVI-1; PEBP2A2; runt domain, alpha subunit 2; runt-related transcription factor 1; RUNX1; SL3/AKV core-binding factor alpha B subunit