Human RUNX1/CBFA2 Antibody

Catalog # Availability Size / Price Qty
AF2399
AF2399-SP
Detection of Human RUNX1/CBFA2 by Western Blot.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human RUNX1/CBFA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RUNX1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human RUNX1
Gly190-Tyr453
Accession # Q01196
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RUNX1/CBFA2 antibody by Western Blot. View Larger

Detection of Human RUNX1/CBFA2 by Western Blot. Western blot shows cytoplasmic and nuclear extracts from Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human RUNX1/CBFA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human RUNX1/CBFA2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human RUNX1/CBFA2 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for RUNX1/ CBFA2 at approximately 59 kDa (as indicated) using 20 µg/mL of Goat Anti-Human RUNX1/CBFA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2399) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX1/CBFA2

RUNX1, also known as CBFA2, is one of three members of the Runt-related family. The RUNX/CBFA proteins form the alpha  subunits that combine with the beta subunit (CBFB) to form the heterodimeric transcription factors. RUNX/CBFA plays important roles in hematopoiesis and may regulate the generation of hematopoietic stem cells from hematogenic endothelial cells. Deregulated expression of CBFA2/RUNX1 is associated with malignant diseases of the hematopoietic system. Human and mouse RUNX/CBFA share 95% amino acid sequence homology.

Long Name
Runt-related Transcription Factor 1
Entrez Gene IDs
861 (Human); 12394 (Mouse); 50662 (Rat)
Alternate Names
Acute myeloid leukemia 1 protein; AML1; AML1acute myeloid leukemia 1; AML1-EVI-1; CBFA2; CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1; Core-binding factor subunit alpha-2; EVI-1; PEBP2A2; runt domain, alpha subunit 2; runt-related transcription factor 1; RUNX1; SL3/AKV core-binding factor alpha B subunit

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human RUNX1/CBFA2 Antibody

There are currently no reviews for this product. Be the first to review Human RUNX1/CBFA2 Antibody and earn rewards!

Have you used Human RUNX1/CBFA2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review