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Human S100A10 Antibody

Catalog #: AF1698
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1698
AF1698-SP
S100A10 in Human Kidney Cancer Tissue.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S100A10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100A10 in direct ELISAs and Western blots. In direct ELISAs, greater than 60% cross-reactivity with recombinant mouse S100A10 and less than 2% cross-reactivity with recombinant human S100P is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human S100A10
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human S100A10
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry S100A10 antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

S100A10 in Human Kidney Cancer Tissue. S100A10 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 5 µg/mL Goat Anti-Human S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1698) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A10

S100A10 is a member of the S100 family of EF hand calcium-binding proteins. It is primarily found in mast cells as heterotetrameric complexes (two annexin A2 subunits and two S100 A10 subunits) that are present intracellularly at the plasma membrane or on the extracellular cell surface. Extracellular S100A10 is a key plasminogen receptor that plays an important role in cellular plasmin production and cellular invasiveness.

Long Name
S100 Calcium Binding Protein
Entrez Gene IDs
6281 (Human); 20194 (Mouse)
Alternate Names
annexin II ligand, calpactin I, light polypeptide; ANX2L; ANX2LG; CAL1LGP11,42C; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; CLP11Ca[1]; MGC111133; p10 protein; p10; P11; protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium binding protein A10; S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium-binding protein A10; S100A10

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Citation for Human S100A10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation
    Authors: SM Thornton, VD Samararatn, JG Skeate, C Buser, KP Lühen, JR Taylor, DM Da Silva, WM Kast
    Cells, 2020-04-15;9(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoelectron Microscopy

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