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Human S100A13 Antibody

Catalog #: AF4327 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4327
AF4327-SP
Detection of Human S100A13 by Western Blot.
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Human S100A13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100A13 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human S100A13
Ala2-Lys98
Accession # Q99584
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human S100A13 antibody by Western Blot. View Larger

Detection of Human S100A13 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. Gels were loaded with 25 µg of cytoplasmic (Cyto) extracts. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human S100A13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4327) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for S100A13 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A13

S100A13 is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-hand Ca++-binding proteins (1‑3). It is widely expressed as a homodimer with two 98 amino acid (aa) long subunits (2, 3). Human S100A13 shares 83%, 90%, 91%, 87%, 78% and 47% aa identity with mouse, rat, cow, dog, opossum and chicken S100A13, respectively. Like other S100 proteins, S100A13 is small and generally acidic, but contains a basic residue-rich sequence at the C terminus, and two EF hand motifs that bind with Ca++ differing affinities (2‑4). Some S100 proteins, including S100A13, are able to bind the cell surface receptor for advanced glycation end-products (RAGE) (5). Despite lacking a signal sequence, S100A13 plays an important role in Cu++-dependent export of FGF-1 (FGF acidic) and IL-1 alpha from the cell in response to stresses such as heat shock, anoxia and starvation (6‑8). Binding of copper is necessary for formation of a multi-protein complex between S100A13, FGF-1 and p40 synaptotagmin-1 (syt-1) (9, 10). Cu++ ions supplied by S100A13 are thought to oxidize and downregulate the activity of FGF-1 prior to export (10). Calcium influx may also play a similar role in FGF-1 release from neuronal cells (11). S100A13 is composed of four amphiphilic helices that may interact with acidic phospholipid headgroups. With FGF-1 and syt-1, S100A13 likely perturbs the membrane, which allows the S100A13 protein complex to exit the cell (4, 12). S100A13 has been proposed as a marker for angiogenesis in tumors and endometrium, due to its role in stress-induced export of FGF-1 (13, 14). Based on in house studies, S100A13 has also been found to promote neurite outgrowth from rat cortical embryonic neurons (15).

References
  1. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.  
  2. Wicki, R. et al. (1996) Biochem. Biophys. Res. Commun. 227:594. 
  3. Ridinger, K. et al. (2000) J. Biol. Chem. 275:8686. 
  4. Li, M. et al. (2007) Biochem. Biophys. Res. Commun. 356:616. 
  5. Hsieh, H.-L. et al. (2004) Biochem. Biophys. Res. Commun. 316:949. 
  6. Landriscina, M. et al. (2001) J. Biol. Chem. 276:22544. 
  7. Sivaraja, V. et al. (2006) Biophys. J. 91:1832.
  8. Mandinova, A. et al. (2003) J. Cell Sci. 116:2687.
  9. Prudovsky, I. et al. (2002) J. Cell Biol. 158:201.
  10. Landriscina, M. et al. (2001) J. Biol. Chem. 276:25549.
  11. Matsunaga, H. and H. Ueda (2006) Cell. Mol. Neurobiol. 26:237.
  12. Graziani, I. et al. (2006) Biochem. Biophys. Res. Commun. 349:192.
  13. Landriscina, M. et al. (2006) J. Neurooncol. 80:251.
  14. Hayrabedyan, S. et al. (2005) Reprod. Biol. 5:51.
  15. R&D Sytems (2007) In-house data.
Long Name
S100 Calcium Binding Protein
Entrez Gene IDs
6284 (Human); 20196 (Mouse); 295213 (Rat)
Alternate Names
S100 calcium binding protein A13; S100 calcium-binding protein A13protein S100-A13; S100A13

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