Home / S1P5/EDG-8 / Human S1P5/EDG-8 Antibody

Human S1P5/EDG-8 Antibody

Catalog #: MAB3964 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3964
MAB3964-SP
Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG‑8 and eGFP by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S1P5/EDG-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S1P5/EDG‑8. Stains human S1P5/EDG‑8 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 282503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human S1P5/EDG‑8
Met1-Asp398
Accession # Q9H228
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µL/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<sub>5</sub>/EDG-8 antibody in HEK293 Human Cell Line Transfected with Human S1P<sub>5</sub>/EDG-8 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Human S1P5/EDG‑8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human S1P5/EDG-8 and eGFP were stained with either (A) Mouse Anti-Human S1P5/EDG-8 Monoclonal Antibody (Catalog # MAB3964) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P5/EDG-8

S1P5 is also known as EDG-8 and nerve growth factor-related G-protein-coupled receptor-1 (NRG-1). S1P5 is a 398 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P5 show 96% and 97% aa identity with mouse and rat S1P5, respectively. Isoform 1 is expressed at a low level in peripheral tissues. Isoform 2 has an alternate C-terminal that is 88 aa shorter and is expressed mainly in brain, spleen, and PBMC. S1P5 is upregulated in large granular lymphocytic leukemias.

Long Name
Endothelial Differentiation Gene 8
Entrez Gene IDs
53637 (Human); 94226 (Mouse); 60399 (Rat)
Alternate Names
EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human S1P5/EDG-8 Antibody

There are currently no reviews for this product. Be the first to review Human S1P5/EDG-8 Antibody and earn rewards!

Have you used Human S1P5/EDG-8 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review