Human SCL/Tal1 Antibody

Catalog #: AF3360
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3360
AF3360-SP

Detection of Human SCL/Tal1 by Western Blot.

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All SCL/Tal1 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SCL/Tal1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SCL in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SCL
Arg199-Gly266
Accession # P17542

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

Immersion fixed HUVEC human umbilical vein endothelial cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SCL/Tal1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human SCL/Tal1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SCL/Tal1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCL/Tal1

SCL, also known as TAL-1 (T-cell Acute Lymphocytic Leukemia-1 protein), is a 40 kDa basic helix-loop-helix transcription factor. It regulates hematopoiesis, angiogenesis and erythrocyte maturation. Activation of SCL by chromosomal translocation may be a cause of some T-cell acute lymphoblastic leukemia. Two alternative splice variants lacking the first 25 or 175 amino acid residues also exist. Human and mouse SCL share 93% amino acid sequence identity.

Long Name

Stem Cell Leukemia/T-cell Acute Lymphocytic Leukemia 1

Entrez Gene IDs

6886 (Human)

Alternate Names

BHLHA17; bHLHa17tal-1; Class A basic helix-loop-helix protein 17; SCL; SCLT-cell acute lymphocytic leukemia protein 1; Stem Cell Protein; Tal1; TAL-1; T-cell acute lymphocytic leukemia 1; T-cell leukemia/lymphoma protein 5; TCL5; TCL5tal-1 product

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human SCL/Tal1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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