Human SDHA Antibody

Catalog #: MAB11586 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11586-100
MAB11586-SP

Save 15% on Select RUO Reagents. See Details.

Detection of Human SDHA by Western Blot.

4 Images

All SDHA Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SDHA Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide corresponding to residues near amino acid 610 of human SDHA protein by Direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1084056

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # P31040

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line

Simple Western

20 µg/mL

HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human kidney

Immunocytochemistry

3-25 µg/mL

Immersion fixed MCF-7 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human SDHA by Western Blot. Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human SDHA Monoclonal Antibody (Catalog # MAB11586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SDHA at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

View Larger

Detection of SDHA in Human Kidney. SDHA was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human SDHA Monoclonal Antibody (Catalog # MAB11586) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry

View Larger

Detection of SDHA in MCF‑7 Human Cell Line. SDHA was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human SDHA Monoclonal Antibody (Catalog # MAB11586) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

View Larger

Detection of Human SDHA by Simple Western^TM. Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 1 mg/ml. A specific band was detected for SDHA at approximately 63 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human SDHA Monoclonal Antibody (Catalog # MAB11586). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SDHA

SDHA is 73 kDa protein located on the inner membrane of the mitochondria which participates in both the citric acid cycle and the respiratory chain. SDHA is one subunit of the SDH complex, which contains 4 total subunits, that are encoded by nuclear genes. All the SDH genes are involved in the tumorigenesis of different types of cancers. Germline SDHA mutations are a driver for multiple types of cancers.

References

Horsefield R, Yankovskaya V, Sexton G, Whittingham W, Shiomi K, Omura S, Byrne B, Cecchini G, Iwata S. Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction. J Biol Chem. 2006 Mar 17;281(11):7309-16. doi: 10.1074/jbc.M508173200. Epub 2005 Dec 27. PMID: 16407191.
Schipani A, Nannini M, Astolfi A, Pantaleo MA. SDHA Germline Mutations in SDH-Deficient GISTs: A Current Update. Genes (Basel). 2023 Mar 4;14(3):646. doi: 10.3390/genes14030646. PMID: 36980917; PMCID: PMC10048394.
Dubard Gault M, Mandelker D, DeLair D, Stewart CR, Kemel Y, Sheehan MR, Siegel B, Kennedy J, Marcell V, Arnold A, Al-Ahmadie H, Modak S, Robson M, Shukla N, Roberts S, Vijai J, Topka S, Kentsis A, Cadoo K, Carlo M, Latham Schwark A, Reznik E, Dinatale R, Hechtman J, Borras Flores E, Jairam S, Yang C, Li Y, Bayraktar EC, Ceyhan-Birsoy O, Zhang L, Kohlman W, Schiffman J, Stadler Z, Birsoy K, Kung A, Offit K, Walsh MF. Germline SDHA mutations in children and adults with cancer. Cold Spring Harb Mol Case Stud. 2018 Aug 1;4(4):a002584. doi: 10.1101/mcs.a002584. PMID: 30068732; PMCID: PMC6071569.

Long Name

SDHA

Entrez Gene IDs

6389 (Human); 66945 (Mouse); 157074 (Rat)

Alternate Names

CMD1GG; flavoprotein subunit of complex II; FP; MC2DN1; NDAXOA; PGL5; PPGL5; SDH1; SDH2; SDHA; SDHF; succinate dehydrogenase complex, subunit A, flavoprotein (Fp)