Human Semaphorin 4A Antibody

Catalog #: AF4694 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4694
AF4694-SP

Detection of Human Semaphorin 4A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Semaphorin 4A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Semaphorin 4A in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse Semaphorin 4A is observed, and less than 1% cross-reactivity with recombinant human (rh) Semaphorin 4B, rhSemaphorin 4C, and rhSemaphorin 4D is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4A
Gly32-His683
Accession # Q9H3S1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Semaphorin 4A by Western Blot. Western blot shows lysates of human mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Semaphorin 4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4694) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4A at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4A

Sema4A (Semaphorin 4A; also Sema B) is a class IV member of the semaphorin family of proteins. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. It reportedly binds to Tim-2 and Plexin D1 and B1. In the immune system, it serves to activate naïve T cells and induce a Th1 phenotype. In the nervous system, it induces growth cone collapse in neurons. Mature human Sema4A is an 80 kDa (predicted) type I transmembrane glycoprotein that is 729 amino acids (aa) in length (aa 33-761). It contains a 651 aa extracellular region (aa 33-683) that is characterized by the presence of one Sema domain (aa 36-494), a PSI region (aa 496-528), and an Ig-like C2-type domain (aa 573-631). The 57 aa cytoplasmic region contains two potential phosphorylation sites at Thr744 and Ser747. There are two potential splice variants. One contains a 37 aa substitution for aa 293-328, while a second contains an alternative start site at Met100 coupled to a deletion of aa 122-154. Sema4A is reportedly cleaved to generate both a monomeric and a dimeric soluble isoforms. Over aa 32-683, human Sema4A shares 86% aa identity with mouse Sema4A.

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Alternate Names

CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A; SEMA4A; SEMAB; Semaphorin 4A; semaphorin-4A; semaphorin-B; SEMB; SEMBFLJ12287