Human Semaphorin 4B Antibody

Catalog #: MAB5485 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5485
MAB5485-SP

Detection of Semaphorin 4B in Human PBMC lymphocytes by Flow Cytometry.

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Human Semaphorin 4B Antibody Summary

Species Reactivity

Human

Specificity

Detects human Semaphorin 4B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Semaphorin 4B is observed.

Source

Monoclonal Mouse IgG_2B Clone # 561416

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4B
Leu39-Glu712
Accession # Q9NPR2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Semaphorin 4B in Human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with (A) Mouse Anti-Human Semaphorin 4B Monoclonal Antibody (Catalog # MAB5485) or (B) isotype control antibody (MAB0041), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane Associated Proteins protocol.

Flow Cytometry

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Detection of Semaphorin 4B in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human Semaphorin 4B Monoclonal Antibody (Catalog # MAB5485, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4B

Semaphorin 4B (Sema4B; also SemaC) is a 95‑100 kDa, class IV member of the Semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids (aa) in length. It contains a 674 aa extracellular region (aa 39‑832) that is characterized by one Sema domain (aa 65‑502), a PSI region (aa 520‑574), and an Ig-like C2-type domain (aa 599‑658). There is one potential soluble splice variant that shows a 29 aa substitution for aa 707‑832. Over aa 39‑712, human Sema4B shares 86% aa identity with mouse Sema4B.

Entrez Gene IDs

10509 (Human); 20352 (Mouse); 293042 (Rat)

Alternate Names

KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B; MGC131831; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B; SEMA4B; SEMAC; Semaphorin 4B; semaphorin-4B; SemC