Human Semaphorin 4C Antibody

Catalog #: MAB6125
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6125
MAB6125-SP

Detection of Human Semaphorin 4C by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Semaphorin 4C Antibody Summary

Species Reactivity

Human

Specificity

Detects human Semaphorin 4C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, 7A, recombinant mouse Semaphorin 3B, 3C, 3E, 3F, 4A, 4B, 4C, 4D, 4F, 4G, 6A, 6B, 6C, 6D, or 7A is observed.

Source

Monoclonal Mouse IgG₁ Clone # 660016

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C
Ala21-Gly663 (predicted)
Accession # Q9C0C4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Semaphorin 4C by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human Semaphorin 4C Monoclonal Antibody (Catalog # MAB6125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Semaphorin 4C at approximately 100 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4C

Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature human Semaphorin 4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length. It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499-551), and an Ig-like C2-type domain (aa 556-644). The cytoplasmic region interacts with PZD-domain containing proteins. There are three potential splice variants. One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36. Over aa 21-663, human Semaphorin 4C shares 85% aa identity with mouse Semaphorin 4C.

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Alternate Names

FLJ20369; KIAA1739; MGC126382; MGC126383; M-Sema F; M-SEMA-F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C; SEMA4C; SEMACL1; Semaf; SEMAI; Semaphorin 4C; semaphorin-4C

Product Datasheets

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Related Research Areas

Citation for Human Semaphorin 4C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1