Human Semaphorin 6C Antibody

Catalog #: MAB2219 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2219
MAB2219-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Semaphorin 6C Antibody Summary

Species Reactivity

Human

Specificity

Detects human Semaphorin 6C in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 6A, 6B, 6D, 7A, recombinant mouse Semaphorin 3C, 3E, 3F, or 6C is observed.

Source

Monoclonal Mouse IgG_2A Clone # 254506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6C
Ala25-Val601
Accession # NP_112175

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Semaphorin 6C

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6C

Semaphorin 6C (Sema6C; previously Sema Y) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1 ‑ 3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that exhibit neuropilin-independent binding to specific plexin A receptors (1 ‑ 3). Sema6C is expressed developmentally in subregions of the central and peripheral nervous systems, heart, and kidney, and primarily in skeletal muscle in adults (3, 4). Human Sema6C cDNA encodes 930 aa, including a 24 aa signal sequence, a 579 aa extracellular domain (ECD) including the Sema domain, a 21 aa transmembrane sequence and a 306 aa cytoplasmic portion. Alternate exon splicing creates a 922 aa short form (Sema6C.3) that is lacking aa 184 - 223 within the Sema domain, but contains 32 unique aa inserted after aa 586; postnatally, this form predominates in muscle (2, 3). A 962 aa form contains only the insert. The 930 aa “long form” predominates in brain, especially in areas of increased plasticity (4). Human Sema6C ECD shares 92%, 93%, 94%, 94%, 95% and 87% aa identity with corresponding mouse, rat, porcine, bovine, equine and canine sequences, respectively. Sema6C, along with Sema6D, is co-expressed with and binds to Plexin A1 (5). This interaction is thought to guide proprioceptive peripheral neurons by repulsion, excluding them from the superficial dorsal horn of the spinal cord (5). Sema6C is down‑regulated and redistributed following denervation or axotomy, potentially promoting regrowth (4, 6). In muscle, Sema6C is concentrated at neuromuscular junctions (6).

References

Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
Burgaya, F. et al. (2006) Mol. Cell. Neurosci. 33:321.
Yoshida, Y. et al. (2006) Neuron 52:775.
Svensson, A. et al. (2008) J. Mol. Hist. 39:5.

Entrez Gene IDs

10500 (Human); 20360 (Mouse)

Alternate Names

KIAA1869; m-SemaY; m-SemaY2; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C; Sema Y; Sema6C; Semaphorin 6C; Semaphorin Y; semaphorin-6C; semaphorin-Y; SEMAY