Human Semaphorin 6D Antibody Summary
Ser22-His660
Accession # Q8NFY4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 6D
Semaphorin 6D (Sema6D) is a ~130-135 kDa member of the Semaphorin family of axon guidance molecules (1-3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that share ~40% amino acid (aa) identity and exhibit neuropilin-independent binding to specific plexin A receptors (1, 2). Sema6D is expressed in the cardiac tube and within the brain and spinal cord during development (2-5). It shows broad expression postnatally, including neurons, lymphocytes, dendritic cells and osteoclasts (5-8). Brain, kidney, placenta, and cardiac and skeletal muscles show highest mRNA expression (2, 5). The primary human Sema6D isoform (1073 aa) includes a 21 aa signal sequence, a 642 aa extracellular domain (ECD) including Sema and PSI domains, a 21 aa transmembrane sequence and a 390 aa cytoplasmic portion. The ECD of this isoform shares 96%, 96%, 98%, 98% and 97% aa identity with corresponding mouse, rat, bovine, equine and canine sequences, respectively. Alternate splicing creates isoforms of 1054, 1030, 1017, 1011 and 998 that lack sequences between aa 570 and 644 and/or contain a 13 aa insert after aa 549; all variations affect the PSI domain (2, 5). An isoform of 476 aa is truncated after the Sema domain (2). All Sema6D isoforms are present in the brain, but are differentially expressed elsewhere (2, 5). In the developing brain, both Sema6D and Sema6C are co-expressed with Plexin A1 and interact differentially to guide proprioceptive peripheral neurons by repulsion (4). Sema6D is essential for morphogenesis of the cardiac ventricle in the chick, signaling both forward through Plexin A1 and in reverse through Sema6D (3, 9). Sema6D mediates survival and anchorage-independent growth of malignant pleural mesothelioma (10) It is active in immune responses by Sema6D+ T cell stimulation of dendritic cells, and in bone homeostasis by engaging osteoclast Plexin A1 (6-8). In all these settings, Sema6D acts through a complex of Plexin A1 with receptor tyrosine kinases such as VEGF R2 (3, 6-10).
- Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
- Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
- Toyofuku, T. et al. (2004) Genes Dev. 18:435.
- Yoshida, Y. et al. (2006) Neuron 52:775.
- Taniguchi, M. and T. Shimizu (2004) Biochem. Biophys. Res. Commun. 314:242.
- O’Connor, B.P. et al. (2008) Proc. Natl. Acad. Sci. USA 105:13015.
- Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
- Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
- Toyofuku, T. et al. (2004) Nat. Cell Biol. 6:1204.
- Catalano, A. et al. (2009) Cancer Res. 69:1485.
Product Datasheets
Citation for Human Semaphorin 6D Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D
Authors: S Demolli, A Doddaballa, K Devraj, K Stark, Y Manavski, A Eckart, CM Zehendner, T Lucas, T Korff, M Hecker, S Massberg, S Liebner, D Kaluza, RA Boon, S Dimmeler
Cardiovasc. Res., 2017-05-01;113(6):681-691.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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