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Human Semaphorin 6D Antibody

Catalog #: AF2095 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2095
AF2095-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Semaphorin 6D Antibody Summary

Species Reactivity
Human
Specificity
Detects human Semaphorin 6D in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Semaphorin 6C is observed, 5% cross-reactivity with rhSemaphorin 6B is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, recombinant mouse (rm) Semaphorin 3E, rmSemaphorin 3F, rhSemaphorin 3A, and rhSemaphorin 7A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6D
Ser22-His660
Accession # Q8NFY4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Semaphorin 6D

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6D

Semaphorin 6D is a type I transmembrane protein belonging to the semaphorin family. Members of the sema family were originally identified as axon guidance molecules. Semaphorin 6D is expressed during development and in adult kidney, brain, placenta, heart and skeletal muscles. Five splice variants of Semaphorin 6D exist.

Entrez Gene IDs
80031 (Human); 214968 (Mouse); 311384 (Rat)
Alternate Names
KIAA1479FLJ11598; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D; Sema6D; Semaphorin 6D; semaphorin-6D

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